List of female queer characters

Lesbian, bisexual, MTF, and more

In fiction

C. S. Lewis. Miss Hardcastle in That Hideous Strength (very negative portrayal of a stereotypical sadistic butch)
Elizabeth A. Lynn. Norres and Sorren in The Northern Girl
Melissa Scott. Trouble and Cerise in Trouble and Her Friends, the protagonists in Night Sky Mine, many more.
Jo Walton. The mother and her lover in Farthing.